Open access poster: Comparison of nitrifying microbial communities of two full-scale membrane bioreactors treating wastewaters from municipal solid waste using 16S rDNA gene amplicon squencing

Abstract


Backgrounds: Landfill leachate is a complex composition containing high levels of ammonia nitrogen. Membrane bioreactor systems (MBRs) are used for this type of complex wastewater treatment, because of their capacity of working at high concentrations levels of suspended solids and high cell retention times.
Objectives: In this study, 16S rRNA gene amplicon sequencing (NGS) combined with
fluorescent in situ hybridization (FISH) were performed to identify detailed changes of nitrifying bacterial communities in activated sludge of two full-scale MBRs treating the waste effluents from landfill leachate treatment systems
Methods: Samples from activated sludge were collected monthly during fifteen months from two MBR systems. The nitrifying bacterial communities were analyzed using 16S rRNA gene amplicon sequencing (NGS) and fluorescence in situ hybridization (qFISH). Distance-based linear models (DISTLM) were applied to investigate models of environmental interpretation of biological variables.
Conclusions: The two MBRs had different nitrifying microbial community compositions. In the MBR treating the waste effluents with high solids anaerobic digestion the ammonia oxidizing community was predominated by the family Nitrosomonadaceae y and the genus Nitrosococcus. In the MBR treating waste effluents with low solids the family Nitrosomonadaceae predominated but the genus Nitrosococcus was present a considerable lower abundances. We investigated models of environmental interpretation of nitrifying variables using of distance-based linear models (DISTLM).

Reference


Barbarroja, P., Moreno-Mesonero, L., Zornoza, A., Fernández-Navarro, J., Alonso, J.L., Muñagorri, F., García, C., Álvarez, C. (2017) Comparison of nitrifying microbial communities of two full-scale membrane bioreactors treating wastewaters from municipal solid wastes using 16S rDNA gene amplicon sequencing. 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.

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